Assay using brain homogenate for measuring the antioxidant activity of biological fluids.

نویسندگان

  • J Stocks
  • J M Gutteridge
  • R J Sharp
  • T L Dormandy
چکیده

1. Fresh ox-brain homogenate prepared under standard conditions at 4°C and stored at 20°C autoxidizes spontaneously and reproducibly when re-heated to 37°C. 2. The preparation can be used for assaying the antioxidant activity of serum and of other biological fluids. The preparatory and assay procedures are described. 3. The antioxidant activity of normal serum, cerebrospinal fluid, synovial fluid and of various known antioxidants have been compared.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Antioxidant potential of Anogeissus latifolia.

Anogeissus latifolia is widely used in the Indian indigenous system of medicine and is reported to contain leucocyanidins and tannoid principles like ellagic acid and its derivatives. In view of its wide use and its chemical composition, this study was aimed at examining the antioxidant activity of the extract of A. latifolia. The extract was studied for total antioxidant capacity, hydrogen-don...

متن کامل

A Review of Laboratory Methods for Measuring Oxidative Stress

Introduction: Antioxidant defense system, including enzymatic and non-enzymatic antioxidants, protects cells from oxidative damage by neutralizing oxidant compounds. Increasing the production of oxidant species and decrease activity of antioxidant system causes oxidative stress. The role of oxidative stress in the pathogenesis of human diseases and strengthening of human antioxidant defense sys...

متن کامل

Antioxidant and Cytotoxic Activities and Phytochemical Analysis of Euphorbia wallichii Root Extract and its Fractions

Euphorbia wallichii a perennial herb growing mainly in Himalayas has been widely used in folk medicines for its medicinal properties. In the present study, the crude methanolic root extract (CME) and its fractions; n-Hexane Fraction (NHF), n-Butanol Fraction (NBF), Chloroform Fraction (CHF), Ethyl acetate Fraction (EAF) and Aqueous Fraction (AQF) of this plant specie were investigated for antio...

متن کامل

Antioxidant and Cytotoxic Activities and Phytochemical Analysis of Euphorbia wallichii Root Extract and its Fractions

Euphorbia wallichii a perennial herb growing mainly in Himalayas has been widely used in folk medicines for its medicinal properties. In the present study, the crude methanolic root extract (CME) and its fractions; n-Hexane Fraction (NHF), n-Butanol Fraction (NBF), Chloroform Fraction (CHF), Ethyl acetate Fraction (EAF) and Aqueous Fraction (AQF) of this plant specie were investigated for antio...

متن کامل

Evaluation of Antioxidant Activity of Methanolic Extract of Elaegnus Angustifolia by Frap Assay

Nowadays the researches have indicated the fact that using synthetic antioxidants as food additives in edible oils can have adverse effects on human health. So preparing antioxidation resources for reduction of oxidation stress effects is important. The aim of this project was, representing a natural antioxidant source with frap test. The present study optimized the ultrasound assisted extracti...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical science and molecular medicine

دوره 47 3  شماره 

صفحات  -

تاریخ انتشار 1974